2023.08.23 Adding immunity to human kidney-on-a-chip

PNAS: Immune-infiltrated Kidney Organoid-on-Chip Model for Assessing T Cell Bispecific Antibodies Kidney organoid-on-chip_Figure: This illustration explains how the immune-infiltrated kidney organoid-on-chip model works: human kidney organoids are seeded into the millifluidic chip device and cultured for seven days under high flow culture conditions. On day seven, peripheral bone marrow-derived immune cells are added to the reservoir with the circulating culture medium, and the chip is continued to be cultured under high flow conditions for three to five days until a wide range of assays can be performed. Credit: Wyss Institute at Harvard University Kidney organoid-on-chip: The team’s study demonstrated in a novel immune-infiltrated human kidney organoid-on-chip model that a T cell bispecific antibody (TCB) targeting an antigen from the Wilms tumor-1 protein (WT1-TCB) specifically recruits immune cells, including cytotoxic T cells (shown in green), to clusters of podocytes (shown in blue), leading to their destruction. The grey staining is derived from dying cells. Credit: Wyss Institute at Harvard University