Towards the quantification of ⍺-synuclein aggregates with digital seed amplification assays

Detecting Parkinson_Fig 4C: Captured ⍺-synuclein aggregates in microwells. Image 2 caption: The researchers showed that minute amounts of ⍺-synuclein aggregates, captured with antibody-coated magnetic beads and extended in vitro, can be precisely measured by counting individual aggregates in microwells. Credit: Wyss Institute at Harvard University Detecting Parkinson_Fig4 AB: Bead-based digital SAA for ⍺-synuclein. Image 1 caption: In this version of their digital seed amplification assay (digital SAA), which first captures ⍺-synuclein aggregates on antibody-coated magnetic beads (illustration on the left), the team demonstrated the subsequent growth of individual ⍺-synuclein fibrils over 150 minutes in vitro (immunofluorescence images on the right). Credit: Wyss Institute at Harvard University